[[Image:Yakiv Ivanovych Ratushnyak 2011.jpg|400px|left]]
===M.Sc., Ph.D===
===Date & Place of birth===
April 5, 1961; Studena, Vinnytsya distr., Ukraine

==Contacts==
{|
|phone:||+380 (44) 266-70-53
|-
|fax:||+380 (44) 252-17-86
|}


==Education & Degrees==
{|
||1981-1986||M.Sc. in Genetics||Thesis: “Genetic complementation of tomates in somatic and sexual hybridization” Kyiv State University, Kyiv, Ukraine
|-
|1986-1991||Ph.D. in Genetics.||Thesis: “Transgenomic plants in Lycopersicon obtained by γ-hybridization of protoplasts” Institute of Cell Biology & Genetic Engineering Academy of Sciences of the Ukraine, Kyiv, Ukraine
|}

==Positions Held==
{|
|1997- present||Senior Researcher||Laboratory of Cytophysiology Institute of Cell Biology & Genetic Engineering National Academy of Sciences of Ukraine, Kyiv, Ukraine
|-
|1997-1998||Postdoctoral Fellow||Laboratory of Prof. Dr. Peter Meyer University of Leeds, Leeds, United Kingdom
|-
|1991-1996||Researcher||Laboratory of Cytophysiology & Cell Engineering Institute of Cell Biology & Genetic Engineering National Academy of Sciences of Ukraine, Kyiv, Ukraine
|-
|1989-1991||Research Assistant||Laboratory of Cytophysiology & Cell Engineering Institute of Cell Biology & Genetic Engineering National Academy of Sciences of Ukraine, Kyiv, Ukraine
|-
|1986-1989||Research Assistant||Laboratory of Cytophysiology & Cell Engineering Institute of Botany National Academy of Sciences of Ukraine, Kyiv, Ukraine
|-
|1983-1986||Research Technician||Laboratory of Cytophysiology & Cell Engineering Institute of Botany National Academy of Sciences of Ukraine, Kyiv, Ukraine
|}

===Teaching experience===
{|
|1987-1990||Teaching Assistant||Principles of Tomato Cell Engineering||Institute of Cell Biology & Genetic Engineering Academy of Sciences of Ukraine, Kyiv, Ukraine
|-
|1992-1997||Teacher||Plant Tissue Culture and Micropropagation||Department of Cell Biology & Genetic Engineering, National University of Kyiv, Kyiv, Ukraine
|}

===Grants===
{|
|1993||Individual Grant from the International Scientific Fund, USA
|-
|1994||Travel Grant from the International Scientific Fund, USA
|-
|1997||Posdoctoral Fellowship from the Royal Society/NATO, UK
|}

==Summary of Research Activities==

As a MSc student, I worked on protoplast culture and plant regeneration of Lycium barbarum (woody bush).

My PhD thesis was devoted to obtaining and analysing transgenomic plants of: L. esculentum with L. peruvianum var. dentatum, L. pennellii, L. hirsutum var. glabratum, Solanum rickii and Lycium barbarum. The results obtained and perspective of future investigations of fertile asymmetric somatic hybrids L. esculentum + L. peruvianum var. dentatum were announced in Rice Biotechnology Quarterly (Gamma Fusion of Tomato Protoplasts, 1991, V 8, p 38-39).

My postdoctoral work started with studies of tomato cybrid plants with chloroplasts of L. peruvianum var. dentatum and reconstructed mitochondria with phenotypic traits of nucleo-cytoplasmic incompatibility.

Then cybrid plants of L. peruvianum var. dentatum were obtained containing tomato chloroplasts and L. peruvianum var. dentatum mitochondria that along with cybrid tomato/peruvianum could serve as an excellent model for studies of nuclear-cytoplasmic and chloroplast-mitochondrial interactions.

Also, by restriction analysis of chloroplast DNA for asymmetric somatic hybrids between L. esculentum and L. peruvianum var. dentatum the presence of the new nonparental patterns was shown. Blot-hybridization of chloroplast DNA with Nicotiana tabacum chloroplast DNA fragments revealed rearrangements which might be interpreted as recombination of chloroplast DNA.

At the same time, for fertile asymmetric somatic hybrids L. esculentum + L. peruvianum var. dentatum a considerable genetic variability was shown. Morphological, cytogenetic, isozyme, ribosomal DNA and random amplified polymorphism DNA analyses of F1 and F2 progenies obtained after self-pollination of somatic hybrids revealed new nonparental patterns of DNA and enzymes, chromosome elimination and variation of leaf morphology.

Also, in collaboration with Prof. C.M.Rick (Tomato Stock Center, Davis, USA) we studied protoplast culture of other species of Petota section S. ochrantum, S. juglandifolium, as well as possibilities of their somatic hybridization with tomato.

Then I worked on genetic transformation of Arabidopsis by leaf-disk and root co-cultivation with Agrobacterium in a project named “Identification, isolation and analysis of stress-induced plants genes in Arabidopsis thaliana by T-DNA tagging and gene fusion” funded by the INTAS. 

Also, I worked on obtaining haploid plants from Petunia cultivars and Nicotiana species (using anther and pollen culture). These are used in activation tagging experiments aimed to achieve genetic transformation by co-cultivation of haploid protoplasts with Agrobacterium. Co-cultivation allowed the selection some PEG- and salt-resistant clones of N. tabacum. These clones might become useful material to isolate salt- and PEG-resistant plants, and to identify genes responsible for salt- and PEG-resistant. I also study possibilities of the use of diploid protoplasts of Arabidopsis thaliana in these experiments. The title of the project is “Activation tagging Arabidopsis thaliana and Petunia hybrida” and it is funded by the Royal Society and NATO.

At present, I am studying possibilities for successful integration of foreing sequences into plastome of some Solanaceae species via PEG-mediated protoplast transformation.

==Scientific Interests==
* organelle DNA recombination and nucleo-cytoplasmic interactions under somatic hybridization in higher plants,
* genetic variability of fertile asymmetric somatic hybrids,
* plant genetic transformation.

===Experience===
Plant cell tissue and protoplast culture, mutagenesis and mutant selection, haploid production, plant genetic transformation (Agrobacterium-mediated procedure, direct gene transfer with and without electroporation, chloroplast transformation), somatic hybridization, biochemical and molecular analyses (DNA-fragment amplification by PCR, isozyme analysis).

==Selected Publications==
# Ratushnyak YI, Piven NM, Rudas VA (1989) Protoplast culture and plant regeneration in Lycium barbarum L. Plant Cell Tissue Organ Cult 17:183-190
# Ratushnyak YI, Piven NM, Rudas VA (1990) Regeneration of Lycium barbarum L. plants from leaf tissue, callus culture and callus protoplasts. Plant Cell Rep 9:84-87
# Ratushnyak YI, Latypov SA, Samoylov AM, Piven NM, Gleba YY (1991) Introgressive hybrydization of tomatoes by "gamma-fusion" of the Lycopersicon esculentum Mill. and Lycopersicon peruvianum var. dentatum Dun. protoplasts. Plant Sci 73:65-78
# Ratushnyak YI, Piven NM, Latypov SA, Samoylov AM, Zavgorodnyaya AV, Gleba YY (1991) Changes of the genetic constitution of the tomato plants by 'gamma-fusion' of Lycopersicon esculentum Mill. and Lycopersicon peruvianum var. dentatum Dun. protoplasts. Biopolymers and Cell (Rus) 7:82-91
# Ratushnyak YI (1991) Gamma fusion of tomato protoplasts. Rice Biotechnology Quarterly 8:38-39
# Ratushnyak YI, Rudas VA, Piven NM (1993) Regeneration of plants from protoplasts of Lycium barbarum L. (Wolfberry). In: Bajaj YPS (ed) Biotechology in Agriculture and Forestry, Vol 22, Plant Protoplasts and Genetic Engineering III, Springer, New York, pp 79-86
# Ratushnyak YI, Cherep NN, Zavgorodnyaya AV, Latypov SA, Borozenko IV, Rachkovskaya RI, Gleba YY (1993) Fertile asymmetric somatic hybrids between Lycopersicon esculentum Mill. and Lycopersicon peruvianum var. dentatum Dun. Mol Gen Genet, 236: 427-432
# Ratushnyak YI, Cherep NN, Zavgorodnyaya AV, Latypov SA, Borozenko IV, Rachkovskaya RI, Gleba YY (1993) Cell engineering synthesis of tomato fertile asymmetric somatic hybrids by "fusion" of protoplasts. Genetics (Russia), 29:164-176
# Ratushnyak YI, Cherep NN, Latypov SA (1994) Somatic hybridization of Lycopersicon esculentum Mill. + Lycopersicon peruvianum var. dentatum Dun. In: Bajaj YPS (ed) Biotechology in Agriculture and Forestry, Vol. 27 Somatic Hybridization in Crop Improvement, Springer, New York, pp 224-243
# Ratushnyak YI, Kochevenko AS, Cherep NN, Zavgorodnyaya AV, Latypov SA, Gleba YY (1995) Alloplasmatic incompatibility in cybrid plants possessing Lycopersicon esculentum Mill. genome and Lycopersicon peruvianum var. dentatum Dun. plasmagenes. Russian Journal of Genetics, 31:565-571
# Kochevenko AS, Ratushnyak YI, Gleba YY (1996) Protoplast culture and somaclonal variability of species of series Juglandifolia. Plant Cell Tissue Organ Cult, 44:103-110 
# Kochevenko AS, Rudas VA, Ratushnyak YI (1999) Analysis chlorophyll-deficiency mutation of Lycopersicon peruvianum var. dentatum by obtain the cytoplasmic hybrids. Russian Journal of Genetics 35:1-8
# Kochevenko AS, Ratushnyak YI, Korneev DU, Stasik OO, Shevchenko VV, Kochubey SM, Gleba YY (1999) Photosynthetic apparatus in cytoplasmic hybrid of cultured tomato carrying nuclear-cytoplasmic incompatibility traits. Russian Journal of Plant Physiology, 46:474-481 

[[Category:Staff|Ratushnyak]]
[[Category:Staff of the Department of genetic engineering|Ratushnyak]]
[[Category:Staff of the Laboratory of Adaptative Biotechnology]]
[[Category:Yakiv Ratushnyak]]

[[uk:Ратушняк Яків Іванович]]

Laboratory of Adaptational Biotechnology

2025-05-05T09:42:15Z

WikiSysop: /* Staff: */

Yakiv Ivanovych Ratushnyak

2025-05-05T09:41:16Z

WikiSysop:

#REDIRECT [[Yakiv Ratushnyak]]

[[Image:Yakiv Ivanovych Ratushnyak 2011.jpg|400px|left]]
===M.Sc., Ph.D===
===Date & Place of birth===
April 5, 1961; Studena, Vinnytsya distr., Ukraine

==Contacts==
{|
|phone:||+380 (44) 266-70-53
|-
|fax:||+380 (44) 252-17-86
|}


==Education & Degrees==
{|
||1981-1986||M.Sc. in Genetics||Thesis: “Genetic complementation of tomates in somatic and sexual hybridization” Kyiv State University, Kyiv, Ukraine
|-
|1986-1991||Ph.D. in Genetics.||Thesis: “Transgenomic plants in Lycopersicon obtained by γ-hybridization of protoplasts” Institute of Cell Biology & Genetic Engineering Academy of Sciences of the Ukraine, Kyiv, Ukraine
|}

==Positions Held==
{|
|1997- present||Senior Researcher||Laboratory of Cytophysiology Institute of Cell Biology & Genetic Engineering National Academy of Sciences of Ukraine, Kyiv, Ukraine
|-
|1997-1998||Postdoctoral Fellow||Laboratory of Prof. Dr. Peter Meyer University of Leeds, Leeds, United Kingdom
|-
|1991-1996||Researcher||Laboratory of Cytophysiology & Cell Engineering Institute of Cell Biology & Genetic Engineering National Academy of Sciences of Ukraine, Kyiv, Ukraine
|-
|1989-1991||Research Assistant||Laboratory of Cytophysiology & Cell Engineering Institute of Cell Biology & Genetic Engineering National Academy of Sciences of Ukraine, Kyiv, Ukraine
|-
|1986-1989||Research Assistant||Laboratory of Cytophysiology & Cell Engineering Institute of Botany National Academy of Sciences of Ukraine, Kyiv, Ukraine
|-
|1983-1986||Research Technician||Laboratory of Cytophysiology & Cell Engineering Institute of Botany National Academy of Sciences of Ukraine, Kyiv, Ukraine
|}

===Teaching experience===
{|
|1987-1990||Teaching Assistant||Principles of Tomato Cell Engineering||Institute of Cell Biology & Genetic Engineering Academy of Sciences of Ukraine, Kyiv, Ukraine
|-
|1992-1997||Teacher||Plant Tissue Culture and Micropropagation||Department of Cell Biology & Genetic Engineering, National University of Kyiv, Kyiv, Ukraine
|}

===Grants===
{|
|1993||Individual Grant from the International Scientific Fund, USA
|-
|1994||Travel Grant from the International Scientific Fund, USA
|-
|1997||Posdoctoral Fellowship from the Royal Society/NATO, UK
|}

==Summary of Research Activities==

As a MSc student, I worked on protoplast culture and plant regeneration of Lycium barbarum (woody bush).

My PhD thesis was devoted to obtaining and analysing transgenomic plants of: L. esculentum with L. peruvianum var. dentatum, L. pennellii, L. hirsutum var. glabratum, Solanum rickii and Lycium barbarum. The results obtained and perspective of future investigations of fertile asymmetric somatic hybrids L. esculentum + L. peruvianum var. dentatum were announced in Rice Biotechnology Quarterly (Gamma Fusion of Tomato Protoplasts, 1991, V 8, p 38-39).

My postdoctoral work started with studies of tomato cybrid plants with chloroplasts of L. peruvianum var. dentatum and reconstructed mitochondria with phenotypic traits of nucleo-cytoplasmic incompatibility.

Then cybrid plants of L. peruvianum var. dentatum were obtained containing tomato chloroplasts and L. peruvianum var. dentatum mitochondria that along with cybrid tomato/peruvianum could serve as an excellent model for studies of nuclear-cytoplasmic and chloroplast-mitochondrial interactions.

Also, by restriction analysis of chloroplast DNA for asymmetric somatic hybrids between L. esculentum and L. peruvianum var. dentatum the presence of the new nonparental patterns was shown. Blot-hybridization of chloroplast DNA with Nicotiana tabacum chloroplast DNA fragments revealed rearrangements which might be interpreted as recombination of chloroplast DNA.

At the same time, for fertile asymmetric somatic hybrids L. esculentum + L. peruvianum var. dentatum a considerable genetic variability was shown. Morphological, cytogenetic, isozyme, ribosomal DNA and random amplified polymorphism DNA analyses of F1 and F2 progenies obtained after self-pollination of somatic hybrids revealed new nonparental patterns of DNA and enzymes, chromosome elimination and variation of leaf morphology.

Also, in collaboration with Prof. C.M.Rick (Tomato Stock Center, Davis, USA) we studied protoplast culture of other species of Petota section S. ochrantum, S. juglandifolium, as well as possibilities of their somatic hybridization with tomato.

Then I worked on genetic transformation of Arabidopsis by leaf-disk and root co-cultivation with Agrobacterium in a project named “Identification, isolation and analysis of stress-induced plants genes in Arabidopsis thaliana by T-DNA tagging and gene fusion” funded by the INTAS. 

Also, I worked on obtaining haploid plants from Petunia cultivars and Nicotiana species (using anther and pollen culture). These are used in activation tagging experiments aimed to achieve genetic transformation by co-cultivation of haploid protoplasts with Agrobacterium. Co-cultivation allowed the selection some PEG- and salt-resistant clones of N. tabacum. These clones might become useful material to isolate salt- and PEG-resistant plants, and to identify genes responsible for salt- and PEG-resistant. I also study possibilities of the use of diploid protoplasts of Arabidopsis thaliana in these experiments. The title of the project is “Activation tagging Arabidopsis thaliana and Petunia hybrida” and it is funded by the Royal Society and NATO.

At present, I am studying possibilities for successful integration of foreing sequences into plastome of some Solanaceae species via PEG-mediated protoplast transformation.

==Scientific Interests==
* organelle DNA recombination and nucleo-cytoplasmic interactions under somatic hybridization in higher plants,
* genetic variability of fertile asymmetric somatic hybrids,
* plant genetic transformation.

===Experience===
Plant cell tissue and protoplast culture, mutagenesis and mutant selection, haploid production, plant genetic transformation (Agrobacterium-mediated procedure, direct gene transfer with and without electroporation, chloroplast transformation), somatic hybridization, biochemical and molecular analyses (DNA-fragment amplification by PCR, isozyme analysis).

==Selected Publications==
# Ratushnyak YI, Piven NM, Rudas VA (1989) Protoplast culture and plant regeneration in Lycium barbarum L. Plant Cell Tissue Organ Cult 17:183-190
# Ratushnyak YI, Piven NM, Rudas VA (1990) Regeneration of Lycium barbarum L. plants from leaf tissue, callus culture and callus protoplasts. Plant Cell Rep 9:84-87
# Ratushnyak YI, Latypov SA, Samoylov AM, Piven NM, Gleba YY (1991) Introgressive hybrydization of tomatoes by "gamma-fusion" of the Lycopersicon esculentum Mill. and Lycopersicon peruvianum var. dentatum Dun. protoplasts. Plant Sci 73:65-78
# Ratushnyak YI, Piven NM, Latypov SA, Samoylov AM, Zavgorodnyaya AV, Gleba YY (1991) Changes of the genetic constitution of the tomato plants by 'gamma-fusion' of Lycopersicon esculentum Mill. and Lycopersicon peruvianum var. dentatum Dun. protoplasts. Biopolymers and Cell (Rus) 7:82-91
# Ratushnyak YI (1991) Gamma fusion of tomato protoplasts. Rice Biotechnology Quarterly 8:38-39
# Ratushnyak YI, Rudas VA, Piven NM (1993) Regeneration of plants from protoplasts of Lycium barbarum L. (Wolfberry). In: Bajaj YPS (ed) Biotechology in Agriculture and Forestry, Vol 22, Plant Protoplasts and Genetic Engineering III, Springer, New York, pp 79-86
# Ratushnyak YI, Cherep NN, Zavgorodnyaya AV, Latypov SA, Borozenko IV, Rachkovskaya RI, Gleba YY (1993) Fertile asymmetric somatic hybrids between Lycopersicon esculentum Mill. and Lycopersicon peruvianum var. dentatum Dun. Mol Gen Genet, 236: 427-432
# Ratushnyak YI, Cherep NN, Zavgorodnyaya AV, Latypov SA, Borozenko IV, Rachkovskaya RI, Gleba YY (1993) Cell engineering synthesis of tomato fertile asymmetric somatic hybrids by "fusion" of protoplasts. Genetics (Russia), 29:164-176
# Ratushnyak YI, Cherep NN, Latypov SA (1994) Somatic hybridization of Lycopersicon esculentum Mill. + Lycopersicon peruvianum var. dentatum Dun. In: Bajaj YPS (ed) Biotechology in Agriculture and Forestry, Vol. 27 Somatic Hybridization in Crop Improvement, Springer, New York, pp 224-243
# Ratushnyak YI, Kochevenko AS, Cherep NN, Zavgorodnyaya AV, Latypov SA, Gleba YY (1995) Alloplasmatic incompatibility in cybrid plants possessing Lycopersicon esculentum Mill. genome and Lycopersicon peruvianum var. dentatum Dun. plasmagenes. Russian Journal of Genetics, 31:565-571
# Kochevenko AS, Ratushnyak YI, Gleba YY (1996) Protoplast culture and somaclonal variability of species of series Juglandifolia. Plant Cell Tissue Organ Cult, 44:103-110 
# Kochevenko AS, Rudas VA, Ratushnyak YI (1999) Analysis chlorophyll-deficiency mutation of Lycopersicon peruvianum var. dentatum by obtain the cytoplasmic hybrids. Russian Journal of Genetics 35:1-8
# Kochevenko AS, Ratushnyak YI, Korneev DU, Stasik OO, Shevchenko VV, Kochubey SM, Gleba YY (1999) Photosynthetic apparatus in cytoplasmic hybrid of cultured tomato carrying nuclear-cytoplasmic incompatibility traits. Russian Journal of Plant Physiology, 46:474-481

Yakiv Ratushnyak

2025-05-05T09:40:26Z

WikiSysop:

[[Image:Yakiv Ivanovych Ratushnyak 2011.jpg|400px|left]]
===M.Sc., Ph.D===
===Date & Place of birth===
April 5, 1961; Studena, Vinnytsya distr., Ukraine

==Contacts==
{|
|phone:||+380 (44) 266-70-53
|-
|fax:||+380 (44) 252-17-86
|}


==Education & Degrees==
{|
||1981-1986||M.Sc. in Genetics||Thesis: “Genetic complementation of tomates in somatic and sexual hybridization” Kyiv State University, Kyiv, Ukraine
|-
|1986-1991||Ph.D. in Genetics.||Thesis: “Transgenomic plants in Lycopersicon obtained by γ-hybridization of protoplasts” Institute of Cell Biology & Genetic Engineering Academy of Sciences of the Ukraine, Kyiv, Ukraine
|}

==Positions Held==
{|
|1997- present||Senior Researcher||Laboratory of Cytophysiology Institute of Cell Biology & Genetic Engineering National Academy of Sciences of Ukraine, Kyiv, Ukraine
|-
|1997-1998||Postdoctoral Fellow||Laboratory of Prof. Dr. Peter Meyer University of Leeds, Leeds, United Kingdom
|-
|1991-1996||Researcher||Laboratory of Cytophysiology & Cell Engineering Institute of Cell Biology & Genetic Engineering National Academy of Sciences of Ukraine, Kyiv, Ukraine
|-
|1989-1991||Research Assistant||Laboratory of Cytophysiology & Cell Engineering Institute of Cell Biology & Genetic Engineering National Academy of Sciences of Ukraine, Kyiv, Ukraine
|-
|1986-1989||Research Assistant||Laboratory of Cytophysiology & Cell Engineering Institute of Botany National Academy of Sciences of Ukraine, Kyiv, Ukraine
|-
|1983-1986||Research Technician||Laboratory of Cytophysiology & Cell Engineering Institute of Botany National Academy of Sciences of Ukraine, Kyiv, Ukraine
|}

===Teaching experience===
{|
|1987-1990||Teaching Assistant||Principles of Tomato Cell Engineering||Institute of Cell Biology & Genetic Engineering Academy of Sciences of Ukraine, Kyiv, Ukraine
|-
|1992-1997||Teacher||Plant Tissue Culture and Micropropagation||Department of Cell Biology & Genetic Engineering, National University of Kyiv, Kyiv, Ukraine
|}

===Grants===
{|
|1993||Individual Grant from the International Scientific Fund, USA
|-
|1994||Travel Grant from the International Scientific Fund, USA
|-
|1997||Posdoctoral Fellowship from the Royal Society/NATO, UK
|}

==Summary of Research Activities==

As a MSc student, I worked on protoplast culture and plant regeneration of Lycium barbarum (woody bush).

My PhD thesis was devoted to obtaining and analysing transgenomic plants of: L. esculentum with L. peruvianum var. dentatum, L. pennellii, L. hirsutum var. glabratum, Solanum rickii and Lycium barbarum. The results obtained and perspective of future investigations of fertile asymmetric somatic hybrids L. esculentum + L. peruvianum var. dentatum were announced in Rice Biotechnology Quarterly (Gamma Fusion of Tomato Protoplasts, 1991, V 8, p 38-39).

My postdoctoral work started with studies of tomato cybrid plants with chloroplasts of L. peruvianum var. dentatum and reconstructed mitochondria with phenotypic traits of nucleo-cytoplasmic incompatibility.

Then cybrid plants of L. peruvianum var. dentatum were obtained containing tomato chloroplasts and L. peruvianum var. dentatum mitochondria that along with cybrid tomato/peruvianum could serve as an excellent model for studies of nuclear-cytoplasmic and chloroplast-mitochondrial interactions.

Also, by restriction analysis of chloroplast DNA for asymmetric somatic hybrids between L. esculentum and L. peruvianum var. dentatum the presence of the new nonparental patterns was shown. Blot-hybridization of chloroplast DNA with Nicotiana tabacum chloroplast DNA fragments revealed rearrangements which might be interpreted as recombination of chloroplast DNA.

At the same time, for fertile asymmetric somatic hybrids L. esculentum + L. peruvianum var. dentatum a considerable genetic variability was shown. Morphological, cytogenetic, isozyme, ribosomal DNA and random amplified polymorphism DNA analyses of F1 and F2 progenies obtained after self-pollination of somatic hybrids revealed new nonparental patterns of DNA and enzymes, chromosome elimination and variation of leaf morphology.

Also, in collaboration with Prof. C.M.Rick (Tomato Stock Center, Davis, USA) we studied protoplast culture of other species of Petota section S. ochrantum, S. juglandifolium, as well as possibilities of their somatic hybridization with tomato.

Then I worked on genetic transformation of Arabidopsis by leaf-disk and root co-cultivation with Agrobacterium in a project named “Identification, isolation and analysis of stress-induced plants genes in Arabidopsis thaliana by T-DNA tagging and gene fusion” funded by the INTAS. 

Also, I worked on obtaining haploid plants from Petunia cultivars and Nicotiana species (using anther and pollen culture). These are used in activation tagging experiments aimed to achieve genetic transformation by co-cultivation of haploid protoplasts with Agrobacterium. Co-cultivation allowed the selection some PEG- and salt-resistant clones of N. tabacum. These clones might become useful material to isolate salt- and PEG-resistant plants, and to identify genes responsible for salt- and PEG-resistant. I also study possibilities of the use of diploid protoplasts of Arabidopsis thaliana in these experiments. The title of the project is “Activation tagging Arabidopsis thaliana and Petunia hybrida” and it is funded by the Royal Society and NATO.

At present, I am studying possibilities for successful integration of foreing sequences into plastome of some Solanaceae species via PEG-mediated protoplast transformation.

==Scientific Interests==
* organelle DNA recombination and nucleo-cytoplasmic interactions under somatic hybridization in higher plants,
* genetic variability of fertile asymmetric somatic hybrids,
* plant genetic transformation.

===Experience===
Plant cell tissue and protoplast culture, mutagenesis and mutant selection, haploid production, plant genetic transformation (Agrobacterium-mediated procedure, direct gene transfer with and without electroporation, chloroplast transformation), somatic hybridization, biochemical and molecular analyses (DNA-fragment amplification by PCR, isozyme analysis).

==Selected Publications==
# Ratushnyak YI, Piven NM, Rudas VA (1989) Protoplast culture and plant regeneration in Lycium barbarum L. Plant Cell Tissue Organ Cult 17:183-190
# Ratushnyak YI, Piven NM, Rudas VA (1990) Regeneration of Lycium barbarum L. plants from leaf tissue, callus culture and callus protoplasts. Plant Cell Rep 9:84-87
# Ratushnyak YI, Latypov SA, Samoylov AM, Piven NM, Gleba YY (1991) Introgressive hybrydization of tomatoes by "gamma-fusion" of the Lycopersicon esculentum Mill. and Lycopersicon peruvianum var. dentatum Dun. protoplasts. Plant Sci 73:65-78
# Ratushnyak YI, Piven NM, Latypov SA, Samoylov AM, Zavgorodnyaya AV, Gleba YY (1991) Changes of the genetic constitution of the tomato plants by 'gamma-fusion' of Lycopersicon esculentum Mill. and Lycopersicon peruvianum var. dentatum Dun. protoplasts. Biopolymers and Cell (Rus) 7:82-91
# Ratushnyak YI (1991) Gamma fusion of tomato protoplasts. Rice Biotechnology Quarterly 8:38-39
# Ratushnyak YI, Rudas VA, Piven NM (1993) Regeneration of plants from protoplasts of Lycium barbarum L. (Wolfberry). In: Bajaj YPS (ed) Biotechology in Agriculture and Forestry, Vol 22, Plant Protoplasts and Genetic Engineering III, Springer, New York, pp 79-86
# Ratushnyak YI, Cherep NN, Zavgorodnyaya AV, Latypov SA, Borozenko IV, Rachkovskaya RI, Gleba YY (1993) Fertile asymmetric somatic hybrids between Lycopersicon esculentum Mill. and Lycopersicon peruvianum var. dentatum Dun. Mol Gen Genet, 236: 427-432
# Ratushnyak YI, Cherep NN, Zavgorodnyaya AV, Latypov SA, Borozenko IV, Rachkovskaya RI, Gleba YY (1993) Cell engineering synthesis of tomato fertile asymmetric somatic hybrids by "fusion" of protoplasts. Genetics (Russia), 29:164-176
# Ratushnyak YI, Cherep NN, Latypov SA (1994) Somatic hybridization of Lycopersicon esculentum Mill. + Lycopersicon peruvianum var. dentatum Dun. In: Bajaj YPS (ed) Biotechology in Agriculture and Forestry, Vol. 27 Somatic Hybridization in Crop Improvement, Springer, New York, pp 224-243
# Ratushnyak YI, Kochevenko AS, Cherep NN, Zavgorodnyaya AV, Latypov SA, Gleba YY (1995) Alloplasmatic incompatibility in cybrid plants possessing Lycopersicon esculentum Mill. genome and Lycopersicon peruvianum var. dentatum Dun. plasmagenes. Russian Journal of Genetics, 31:565-571
# Kochevenko AS, Ratushnyak YI, Gleba YY (1996) Protoplast culture and somaclonal variability of species of series Juglandifolia. Plant Cell Tissue Organ Cult, 44:103-110 
# Kochevenko AS, Rudas VA, Ratushnyak YI (1999) Analysis chlorophyll-deficiency mutation of Lycopersicon peruvianum var. dentatum by obtain the cytoplasmic hybrids. Russian Journal of Genetics 35:1-8
# Kochevenko AS, Ratushnyak YI, Korneev DU, Stasik OO, Shevchenko VV, Kochubey SM, Gleba YY (1999) Photosynthetic apparatus in cytoplasmic hybrid of cultured tomato carrying nuclear-cytoplasmic incompatibility traits. Russian Journal of Plant Physiology, 46:474-481 

[[Category:Staff|Ratushnyak]]

[[uk:Ратушняк Яків Іванович]]
[[ru:Ратушняк Яков Иванович]]

2024-12-27T13:11:16Z

WikiSysop: WikiSysop moved page Ljudmila Lioshyna to Ljudmyla Lioshyna: 2024-12-27

[[Image:Lioshyna 2016.jpg|left]]

a.k.a. Ljudmila G. Lioshyna 
Ph.D., 
Senior Research Scientist

===Contacts===
llioshina[[Image:At 000099 16x17x2.gif]]ukr.net
llioshina[[Image:At 000099 16x17x2.gif]]icbge.org.ua
llioshyna[[Image:At 000099 16x17x2.gif]]gmail.com

===Areas of interest===
<div style='margin-right:40px;margin-bottom:40px;display:inline-block;vertical-align:top;width:250px;'>
* Biotechnology
* Plant Cell and Molecular Biology
* Genetic Engineering
* Plant Secondary Metabolism
* Microbiology
</div>

===Research Skills:===

Extensive knowledge of molecular and cellular biology, biotechnology

==Selected Publications (peer reviewed)==

# Л.Г.Льошина, Т.В.Медвєдєва, О.В.Булко, А.П.Галкин, В.П.Кухар. Органоспецифічна та світлозалежна експресія генів в трансгенних рослинах: одержання та клонування корене- бульбо- та листспецифічних промоторів // Біополімери та клітина.- 2003.- т.19.-.№2.- С.169-178. https://dx.doi.org/10.7124/bc.00064A
# А.П.Галкин, Л.Г.Лёшина, Т.В.Медведева, О.В.Булко, В.П.Кухар Регуляторные области промоторов генов растений и белки-регуляторы промоторной активности // Биополимеры и клетка.- 2004.- т.20.-.№5.- С.363-379. https://dx.doi.org/10.7124/bc.0006BE
# L.G.Lioshina Cellular and molecular-genetic mechanisms of symbiosis and associative interaction of microorganisms with plants in rhizosphere // Biopolimers and cells.-2009.-25 (1).-P.27-38. https://dx.doi.org/10.7124/bc.0007C7
# Л.Г.Льошина Особливості культивування і ростові характеристики калусної та суспензійної культури клітин Digitalis purpurea L. // Фізіологія рослин: проблеми та перспективи розвитку – К.:Логос, 2009, т.2.- с.607-612
# Лёшина Л.Г., Булко О.В. Agrobacterium rhizogenes-опосредованная трансформация и регенерация двух видов растений из семейства Apocynaceae //Фізіологія та біохімія культурних рослин – 2011-т.43.№6 –с. 533-539.
# L.G.Lioshina, O.V.Bulko Plant regeneration from hairy roots and calluses of periwinkle Vinca minor L. and foxglove purple Digitalis purpurea L. // Cytology and Genetics. – 2014. -Vol.48, №5. - Р.302-307. https://doi.org/10.3103/S009545271405003X
# S.Rogalsky, K.Fatyeyeva, L.Lyoshina, O.Tarasyuk, O.Bulko, S.Lobok Antimicrobial properties and thermal stability of polycarbonate modified with 1-alkyl-3-methylimidazolium tetrafluoroborate ionic liquids - Journal of Applied Polymer Sciencе – 2014. - Vol.131. - Issue 7 - APP.40050. https://doi.org/10.1002/app.40050
# S. Rogalsky, J.-F. Bardeau, H.Wu, L. Lyoshina, O. Bulko, O. Tarasyuk, S. Makhno, T. Cherniavska, Y. Kyselov, J. H. Koo Structural, thermal and antibacterial properties of polyamide 11/polymeric biocide polyhexamethylene guanidine dodecylbenzenesulfonate composites // J Mater Sci – 2016. –V. 51. – P.7716–7730. https://doi.org/10.1007/s10853-016-0054-x
# Lioshyna L., Bulko O., Litvinov S., Pchelovska S., Sokolova D., Berestyanaya A., Tonkal L., Salivon A. X-ray exposure to the stress response from Ri-transformed regenerants Digitalis purpurea L in vitro // RadJ.- 2017.-V.2, N 1.- pp. 1 – 4. https://doi.org/10.21175/RadJ.2017.01.001
# Шиліна Ю.В., Кравець О.А., Льошина Л.Г., Булко О.В., Пчеловська С.А., Літвінов С.В., Жук В.В., Листван К.В., Соколова Д.О., Салівон А.Г.,Тонкаль Л.В. Радіація і вторинний метаболізм рослин // - Київ: Видавничий дім «Кондор»,2019.-130c. http://www.nas.gov.ua/UA/Book/Pages/default.aspx?BookID=0000015869
# Lyoshyna L., Tarasyuk O., Bulko O., Rogalsky S., Kamenieva T., Kuchuk M. Effect of polymeric biocide polyhexamethylene guanidine hydrochloride on morpho-physiological and biochemical parameters of wheat seedlings under copper stress // Agricultural Science and Practice. –2020.-Vol.7,No.1.-pp.49-58.https://doi.org/10.15407/agrisp7.01
# Rogalsky S., Bardeau J.-F., Lyoshina L., Tarasyuk O., Bulko O., Dzhuzha O., Cherniavska T., Kremenitsky V., Kobrina L., Riabov S. New promising antimicrobial material based on thermoplastic polyurethane modified with polymeric biocide polyhexamethylene guanidine hydrochloride // Materials Chemistry and Physics.-2021.-124682,ISSN 0254-0584. https://doi.org/10.1016/j.matchemphys.2021.124682
# Litvinov S.V., Lioshyna L.G., Bulko O.V., Lystvan K.V., Pchelovska S.A. Changes in the content of carotenoids and flavonoids in medicinal raw material of Digitalis purpurea, Polemonium caeruleum, and Aerva lanata, cultivated in vitro under the chronic action of ionizing radiation // Nucl. Phys. At. Energy.- 2021, vol. 22, No 1, pp. 85-92. https://doi.org/10.15407/jnpae2021.01.085
# Loshyna L., Bulko O., Kuchuk M. Adventitious regeneration of blackberry and raspberry shoots and the assessment of the LED-lighting impact //Zemdirbyste-Agriculture.-2022, vol. 109, No. 1, p. 49–54. https://doi.org/10.13080/z-a.2022.109.007 http://www.zemdirbyste-agriculture.lt/wp-content/uploads/2022/03/109_1_str7.pdf
# Rogalsky S.P., Tarasyuk O.P., Bulko O.V., Lioshyna L.G. 2023. Evaluation of growth-promoting effect of 1-dodecyloxycarbonylmethyl-pyridinium chloride on wheat seedlings // Ukr. Bioorg. Acta. - 2023, 18 (2), 47-50. https://doi.org/10.15407/bioorganica2023.02.041

[[Category:Staff|Lioshyna]]
[[Category:Staff of the Department of genetic engineering|Lioshyna]]

[[uk:Льошина Людмила Георгіївна]]

Ljudmila Lioshyna

2024-12-27T13:11:16Z

WikiSysop: WikiSysop moved page Ljudmila Lioshyna to Ljudmyla Lioshyna: 2024-12-27

#REDIRECT [[Ljudmyla Lioshyna]]

2024-12-27T11:33:33Z

WikiSysop:

[[Image:Lioshyna 2016.jpg|left]]

a.k.a. Ljudmila G. Lioshyna 
Ph.D., 
Senior Research Scientist

E-mail:
llioshina[[Image:At 000099 16x17x2.gif]]ukr.net
llioshina[[Image:At 000099 16x17x2.gif]]icbge.org.ua

{{-}}

===Research Skills:===

Extensive knowledge of molecular and cellular biology, biotechnology

==Selected Publications (peer reviewed)==

# Л.Г.Льошина, Т.В.Медвєдєва, О.В.Булко, А.П.Галкин, В.П.Кухар. Органоспецифічна та світлозалежна експресія генів в трансгенних рослинах: одержання та клонування корене- бульбо- та листспецифічних промоторів // Біополімери та клітина.- 2003.- т.19.-.№2.- С.169-178. https://dx.doi.org/10.7124/bc.00064A
# А.П.Галкин, Л.Г.Лёшина, Т.В.Медведева, О.В.Булко, В.П.Кухар Регуляторные области промоторов генов растений и белки-регуляторы промоторной активности // Биополимеры и клетка.- 2004.- т.20.-.№5.- С.363-379. https://dx.doi.org/10.7124/bc.0006BE
# L.G.Lioshina Cellular and molecular-genetic mechanisms of symbiosis and associative interaction of microorganisms with plants in rhizosphere // Biopolimers and cells.-2009.-25 (1).-P.27-38. https://dx.doi.org/10.7124/bc.0007C7
# Л.Г.Льошина Особливості культивування і ростові характеристики калусної та суспензійної культури клітин Digitalis purpurea L. // Фізіологія рослин: проблеми та перспективи розвитку – К.:Логос, 2009, т.2.- с.607-612
# Лёшина Л.Г., Булко О.В. Agrobacterium rhizogenes-опосредованная трансформация и регенерация двух видов растений из семейства Apocynaceae //Фізіологія та біохімія культурних рослин – 2011-т.43.№6 –с. 533-539.
# L.G.Lioshina, O.V.Bulko Plant regeneration from hairy roots and calluses of periwinkle Vinca minor L. and foxglove purple Digitalis purpurea L. // Cytology and Genetics. – 2014. -Vol.48, №5. - Р.302-307. https://doi.org/10.3103/S009545271405003X
# S.Rogalsky, K.Fatyeyeva, L.Lyoshina, O.Tarasyuk, O.Bulko, S.Lobok Antimicrobial properties and thermal stability of polycarbonate modified with 1-alkyl-3-methylimidazolium tetrafluoroborate ionic liquids - Journal of Applied Polymer Sciencе – 2014. - Vol.131. - Issue 7 - APP.40050. https://doi.org/10.1002/app.40050
# S. Rogalsky, J.-F. Bardeau, H.Wu, L. Lyoshina, O. Bulko, O. Tarasyuk, S. Makhno, T. Cherniavska, Y. Kyselov, J. H. Koo Structural, thermal and antibacterial properties of polyamide 11/polymeric biocide polyhexamethylene guanidine dodecylbenzenesulfonate composites // J Mater Sci – 2016. –V. 51. – P.7716–7730. https://doi.org/10.1007/s10853-016-0054-x
# Lioshyna L., Bulko O., Litvinov S., Pchelovska S., Sokolova D., Berestyanaya A., Tonkal L., Salivon A. X-ray exposure to the stress response from Ri-transformed regenerants Digitalis purpurea L in vitro // RadJ.- 2017.-V.2, N 1.- pp. 1 – 4. https://doi.org/10.21175/RadJ.2017.01.001
# Шиліна Ю.В., Кравець О.А., Льошина Л.Г., Булко О.В., Пчеловська С.А., Літвінов С.В., Жук В.В., Листван К.В., Соколова Д.О., Салівон А.Г.,Тонкаль Л.В. Радіація і вторинний метаболізм рослин // - Київ: Видавничий дім «Кондор»,2019.-130c. http://www.nas.gov.ua/UA/Book/Pages/default.aspx?BookID=0000015869
# Lyoshyna L., Tarasyuk O., Bulko O., Rogalsky S., Kamenieva T., Kuchuk M. Effect of polymeric biocide polyhexamethylene guanidine hydrochloride on morpho-physiological and biochemical parameters of wheat seedlings under copper stress // Agricultural Science and Practice. –2020.-Vol.7,No.1.-pp.49-58.https://doi.org/10.15407/agrisp7.01
# Rogalsky S., Bardeau J.-F., Lyoshina L., Tarasyuk O., Bulko O., Dzhuzha O., Cherniavska T., Kremenitsky V., Kobrina L., Riabov S. New promising antimicrobial material based on thermoplastic polyurethane modified with polymeric biocide polyhexamethylene guanidine hydrochloride // Materials Chemistry and Physics.-2021.-124682,ISSN 0254-0584. https://doi.org/10.1016/j.matchemphys.2021.124682
# Litvinov S.V., Lioshyna L.G., Bulko O.V., Lystvan K.V., Pchelovska S.A. Changes in the content of carotenoids and flavonoids in medicinal raw material of Digitalis purpurea, Polemonium caeruleum, and Aerva lanata, cultivated in vitro under the chronic action of ionizing radiation // Nucl. Phys. At. Energy.- 2021, vol. 22, No 1, pp. 85-92. https://doi.org/10.15407/jnpae2021.01.085
# Loshyna L., Bulko O., Kuchuk M. Adventitious regeneration of blackberry and raspberry shoots and the assessment of the LED-lighting impact //Zemdirbyste-Agriculture.-2022, vol. 109, No. 1, p. 49–54. https://doi.org/10.13080/z-a.2022.109.007 http://www.zemdirbyste-agriculture.lt/wp-content/uploads/2022/03/109_1_str7.pdf
# Rogalsky S.P., Tarasyuk O.P., Bulko O.V., Lioshyna L.G. 2023. Evaluation of growth-promoting effect of 1-dodecyloxycarbonylmethyl-pyridinium chloride on wheat seedlings // Ukr. Bioorg. Acta. - 2023, 18 (2), 47-50. https://doi.org/10.15407/bioorganica2023.02.041

[[Category:Staff|Lioshyna]]

[[uk:Льошина Людмила Георгіївна]]