Effect of transformation on bioactive compounds accumulation in Bidens pilosa and Artemisia tilesii "hairy" root culture / Matvieieva N.A., Drobot K.O. 2015

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'''[[Matvieieva N.A.]], [[Drobot K.O.]]'''
 
'''[[Matvieieva N.A.]], [[Drobot K.O.]]'''
  
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Aims.  Genetic  transformation  can  lead  to  physiological  and  biochemical  changes  in  plant  cells  and,  therefore,  changes in  the  synthesis/accumulation  of  natural  compounds  and  the  one  can  be  viewed  as  a  stress  factor.  Study  of  the  effect  of transformation  on  biologically  active  compounds  accumulation  is  of  special  interest  because  transgenic  plants  of  “hairy” root  culture  can  be  used  as  a  source  of  valuable  substances.  Evaluation  of  the  effect  of  genetic  transformation  on  fructan (F),  total  soluble  protein  (TSP)  accumulation  and  antioxidant  activity  (aOa)  of  A.  tilesii  and  B.  pilosa  “hairy”  root  extracts was  the  aim  of  the  work.  Methods.  We  used  A.  tilesii  and  B.  pilosa  “hairy”  root  culture  constructed  by  Agrobacterium rhizogenes  a4-mediated  transformation  (vectors  with  human  ifn-б2b and  nptII  genes).  To  determine  fructans  content  in the  extracts  Selivaniv  method  was  used.  aOa  of  extracts  was  measured  by  DPPH  method.  Bradford  method  was  used  for TSP determine.  Results.  In  some  cases  transformation  have  led  to  the  reduction  of  TSP  and  F  accumulation  in  A.  tilesii  and B.  pilosa  “hairy”  roots.  However,  TSP  and  F  content  in  some  A.  tilesii  and  B.  pilosa  “hairy”  root  lines  was  higher  than  in  the control.  For  example,  TSР  accumulation  in  root  line  №  1  was  8.2  ±  0.5  mg/g  and  this  parameter  was  1.8  time  higher  than the  one  in  the  roots  of  control  plants.  F  content  in  B.  pilosa  root  line  was  higher  than  in  the  control  –  15.5  ±  1.44  mg/g  and 10.07  ±  1.0  mg/g  respectively.  A.  tilesii  and  B.  pilosa  aOa  in  control  roots  was  45.7  %  and  43.25  %  respectively.  aOa  of extracts  of  transgenic  roots  varied  from  30.94  to  51.09  %  and  in  some  cases  it  was  higher  than  aOa  of  extracts  from  the roots  of  the  control  plants.  Conclusions.  Thus,  genetic  transformation  resulted  in  changes  in  TSP,  fructan  content  and  aOa activity  in  Bidens  pilosa  and  Artemisia  tilesii  “hairy”  roots.  In  several  root  lines  we  confirmed  increase  of  aOa,  TSP  and fructans content.  
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Aims.  Genetic  transformation  can  lead  to  physiological  and  biochemical  changes  in  plant  cells  and,  therefore,  changes in  the  synthesis/accumulation  of  natural  compounds  and  the  one  can  be  viewed  as  a  stress  factor.  Study  of  the  effect  of transformation  on  biologically  active  compounds  accumulation  is  of  special  interest  because  transgenic  plants  of  “hairy” root  culture  can  be  used  as  a  source  of  valuable  substances.  Evaluation  of  the  effect  of  genetic  transformation  on  fructan (F),  total  soluble  protein  (TSP)  accumulation  and  antioxidant  activity  (aOa)  of  A.  tilesii  and  B.  pilosa  “hairy”  root  extracts was  the  aim  of  the  work.  Methods.  We  used  A.  tilesii  and  B.  pilosa  “hairy”  root  culture  constructed  by  Agrobacterium rhizogenes  a4-mediated  transformation  (vectors  with  human  ifn-α2b and  nptII  genes).  To  determine  fructans  content  in the  extracts  Selivaniv  method  was  used.  aOa  of  extracts  was  measured  by  DPPH  method.  Bradford  method  was  used  for TSP determine.  Results.  In  some  cases  transformation  have  led  to  the  reduction  of  TSP  and  F  accumulation  in  A.  tilesii  and B.  pilosa  “hairy”  roots.  However,  TSP  and  F  content  in  some  A.  tilesii  and  B.  pilosa  “hairy”  root  lines  was  higher  than  in  the control.  For  example,  TSР  accumulation  in  root  line  №  1  was  8.2  ±  0.5  mg/g  and  this  parameter  was  1.8  time  higher  than the  one  in  the  roots  of  control  plants.  F  content  in  B.  pilosa  root  line  was  higher  than  in  the  control  –  15.5  ±  1.44  mg/g  and 10.07  ±  1.0  mg/g  respectively.  A.  tilesii  and  B.  pilosa  aOa  in  control  roots  was  45.7  %  and  43.25  %  respectively.  aOa  of extracts  of  transgenic  roots  varied  from  30.94  to  51.09  %  and  in  some  cases  it  was  higher  than  aOa  of  extracts  from  the roots  of  the  control  plants.  Conclusions.  Thus,  genetic  transformation  resulted  in  changes  in  TSP,  fructan  content  and  aOa activity  in  Bidens  pilosa  and  Artemisia  tilesii  “hairy”  roots.  In  several  root  lines  we  confirmed  increase  of  aOa,  TSP  and fructans content.  
  
 
Keywords: Bidens pilosa  L.,  Artemisia tilesii  Ledeb., “hairy” root culture,  AOA, fructans,  TSP.
 
Keywords: Bidens pilosa  L.,  Artemisia tilesii  Ledeb., “hairy” root culture,  AOA, fructans,  TSP.
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[[Category:Nadiia Matvieieva]]
 
[[Category:Nadiia Matvieieva]]
 
[[Category:Kateryna Drobot]]
 
[[Category:Kateryna Drobot]]
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[[Category:genetic transformation]]
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[[Category:Agrobacterium rhizogenes]]
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[[Category:Bidens pilosa]]
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[[Category:Artemisia tilesii]]
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[[Category:hair roots]]
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[[Category:ifn-α2b gene]]
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[[Category:nptII gene]]
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[[Category:antioxidant activity]]
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[[uk:Вплив трансформації на накопичення біологічно активних сполук у «бородатих» коренях Bidens pilosa та Artemisia tilesii / Матвєєва Н.А., Дробот К.О. 2015]]
 
[[uk:Вплив трансформації на накопичення біологічно активних сполук у «бородатих» коренях Bidens pilosa та Artemisia tilesii / Матвєєва Н.А., Дробот К.О. 2015]]

Latest revision as of 17:03, 16 December 2015

Matvieieva N.A., Drobot K.O.

Aims. Genetic transformation can lead to physiological and biochemical changes in plant cells and, therefore, changes in the synthesis/accumulation of natural compounds and the one can be viewed as a stress factor. Study of the effect of transformation on biologically active compounds accumulation is of special interest because transgenic plants of “hairy” root culture can be used as a source of valuable substances. Evaluation of the effect of genetic transformation on fructan (F), total soluble protein (TSP) accumulation and antioxidant activity (aOa) of A. tilesii and B. pilosa “hairy” root extracts was the aim of the work. Methods. We used A. tilesii and B. pilosa “hairy” root culture constructed by Agrobacterium rhizogenes a4-mediated transformation (vectors with human ifn-α2b and nptII genes). To determine fructans content in the extracts Selivaniv method was used. aOa of extracts was measured by DPPH method. Bradford method was used for TSP determine. Results. In some cases transformation have led to the reduction of TSP and F accumulation in A. tilesii and B. pilosa “hairy” roots. However, TSP and F content in some A. tilesii and B. pilosa “hairy” root lines was higher than in the control. For example, TSР accumulation in root line № 1 was 8.2 ± 0.5 mg/g and this parameter was 1.8 time higher than the one in the roots of control plants. F content in B. pilosa root line was higher than in the control – 15.5 ± 1.44 mg/g and 10.07 ± 1.0 mg/g respectively. A. tilesii and B. pilosa aOa in control roots was 45.7  % and 43.25  % respectively. aOa of extracts of transgenic roots varied from 30.94 to 51.09  % and in some cases it was higher than aOa of extracts from the roots of the control plants. Conclusions. Thus, genetic transformation resulted in changes in TSP, fructan content and aOa activity in Bidens pilosa and Artemisia tilesii “hairy” roots. In several root lines we confirmed increase of aOa, TSP and fructans content.

Keywords: Bidens pilosa L., Artemisia tilesii Ledeb., “hairy” root culture, AOA, fructans, TSP.

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