Liudmyla Sakhno
From ICBGE
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| + | ==Areas of expertise== | ||
| + | |||
| + | Plant biotechnology; plant physiology; transgenic plants; heterologous gene expression; plant tolerance to abiotic and biotic stresses | ||
| + | |||
| + | ==Major accomplishments== | ||
| + | * Identified transgenic canola lines with higher biomass production under normal conditions as well as in water deficit or heat stress. | ||
| + | * Obtained with colleagues data about physiological, biochemical, and molecular biological characteristics of transgenic canola plants. | ||
| + | * Obtained some groups of transgenic canola plants bearing heterologous genes of different origin such as: | ||
| + | *# BAR gene providing plant tolerance to herbicides with phosphinotricin as acting agent; | ||
| + | *# synthetic EPSPS gene which is responsible for plant resistance to glyphosat herbicides; | ||
| + | *# human interferon alpha 2b gene, HuINFα2b gene; | ||
| + | *# desaturase С gene from cyanobacterium Synocococcus vulcanus, fused in reading frame with repoter lichenase gene from Clostridium thermocellum, DesC::LicBM3 gene; | ||
| + | *# СYP11A1 gene from bovine adrenal cortex mitochondria encoding cytochrome P450SCC. | ||
| + | * Developed a protocol for canola genetic transformation using leaves of aseptic plants as the explants. | ||
| + | * Participated in creation of plant germplasm bank of world flora in part of obtaining of in vitro cultures that was included by the decision of Ukrainian government to the list of scientific objects which are considered as national scientific property. | ||
| + | * Presented scientific results at seminars and meetings. | ||
| + | * Participated in International Conferences in Toruń (Poland), Radzikow (Poland), Łódź (Poland), Krakow (Poland), Moscow (Russia), Yalta (Ukraine), Kyiv (Ukraine). | ||
| + | |||
Revision as of 15:16, 13 October 2014
Liudmyla O. Sakhno
PhD
Lead Senior Scientist
Department of Genetic Engineering
Institute of Cell Biology and Genetic Engineering (ICBGE) NAS of Ukraine
Akademika Zabolotnoho Street, 148, Kyiv 03143, Ukraine
sakhno
icbge.org.ua
Profile on Google Scholar
Contents |
Education
| ICBGE NAS of Ukraine | Ph.D | (Major: Biotechnology) | 2003 |
| Taras Shevchenko Kyiv State University | M.Sci | (Major: Plant physiology) | 1984 |
Appointments
| Lead Senior Scientist | Dept. Genetic Engineering, ICBGE NAS of Ukraine, |
2013-Present |
| Deputy Chair | Dept. Genetic Engineering, ICBGE NAS of Ukraine |
2010 - Present |
| Senior Scientist | Dept. Genetic Engineering, ICBGE NAS of Ukraine |
2008-2013 |
| Research Scientist | Dept. Genetic Engineering, ICBGE NAS of Ukraine |
2003-2008 |
| Assistant Research Scientist | Dept. Genetic Engineering, ICBGE NAS of Ukraine |
1989-2003 |
| Assistant Research Scientist | Dept. Cell Biology and Genetic Engineering, Institute of Botany NAS of Ukraine |
1986-1989 |
| Engineer-biologist | Central Selection and Genetics Station, Uman (Cherkasy region) |
1984-1986 |
Areas of expertise
Plant biotechnology; plant physiology; transgenic plants; heterologous gene expression; plant tolerance to abiotic and biotic stresses
Major accomplishments
- Identified transgenic canola lines with higher biomass production under normal conditions as well as in water deficit or heat stress.
- Obtained with colleagues data about physiological, biochemical, and molecular biological characteristics of transgenic canola plants.
- Obtained some groups of transgenic canola plants bearing heterologous genes of different origin such as:
- BAR gene providing plant tolerance to herbicides with phosphinotricin as acting agent;
- synthetic EPSPS gene which is responsible for plant resistance to glyphosat herbicides;
- human interferon alpha 2b gene, HuINFα2b gene;
- desaturase С gene from cyanobacterium Synocococcus vulcanus, fused in reading frame with repoter lichenase gene from Clostridium thermocellum, DesC::LicBM3 gene;
- СYP11A1 gene from bovine adrenal cortex mitochondria encoding cytochrome P450SCC.
- Developed a protocol for canola genetic transformation using leaves of aseptic plants as the explants.
- Participated in creation of plant germplasm bank of world flora in part of obtaining of in vitro cultures that was included by the decision of Ukrainian government to the list of scientific objects which are considered as national scientific property.
- Presented scientific results at seminars and meetings.
- Participated in International Conferences in Toruń (Poland), Radzikow (Poland), Łódź (Poland), Krakow (Poland), Moscow (Russia), Yalta (Ukraine), Kyiv (Ukraine).
