Перегляд вихідного коду сторінки The positive effect of antibiotic paromomycin compared with kanamycin for selection of transgenic plants with NPTII gene on the example of Nicotiana tabacum / Nitovska I.O. et al. 2015

'''The positive effect of antibiotic paromomycin compared with kanamycin for selection of transgenic plants with NPTII gene on the example of ''Nicotiana tabacum'''''

''[[Nitovska I.O.]], [[Avilov I.D.]], [[Morgun B.V.]]''

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|Fig.  1.  The  effect  of  kanamycin  (a)  and  paromomycin  (B)  on  leaf  explants  of  the  nontransformed  tobacco  plants.  The arrows indicate on regenerated explants
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'''Aims.'''  To  investigate  the  efficiency  of  antibiotic  paromomycin  as  a  selective  agent  for  the  production  of  transgenic  plants with  nptII  gene  compared  with  kanamycin  sulfate  on  the  example  of  Nicotiana  tabacum.  '''Methods.'''  a  vector  containing the  nptII  gene  under  the  control  of  35S  promoter  of  cauliflower  mosaic  virus  was  used  in  Agrobacterium-mediated  genetic transformation  of  leaf  explants  from  Nicotiana  tabacum.  Following  parallel  explant  selection  on  media  with  paromomycin or  kanamycin  plant  regeneration  was  observed.  Total  DNa  of  the  regenerated  plants  was  tested  for  the  presence  of  nptII gene  by  PCr.  '''Results.'''  It  has  been  shown  that  the  use  of  antibiotic  paromomycin  is  effective  for  obtaining  transgenic tobacco  plants.  Paromomycin  revealed  a  softer  effect  on  plant  explants  compared  with  kanamycin  sulfate  without  causing severe  depression  on  regenerative  ability  of  plant  tissues,  leading  to  a  higher  frequency  of  regeneration  on  selective medium  and  transformation.  '''Conclusions.'''  Transgenic  tobacco  plants  were  received  on  selective  media  containing paromomycin  with  high  frequency.  Due  to  the  mild  effects  on  plant  explants,  paromomycin  is  an  attractive  agent  for selection  of  transgenic  plants  with  nptII  gene,  especially  for  those  species  in  which  the  kanamycin  sulfate  has  a  strong negative impact on the regenerative potential. 

'''Keywords:''' Paromomycin, kanamycin,  nptII,  Nicotiana tabacum, selection of transgenic plants.


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| colspan=2| Fig.  2.  Electrophoretic  analysis  of  the  PCr  products  from  tobacco  regenerant  lines  for  the  presence  of  nptII  (a)  and vir  D1  (B)  genes.  Lanes  25–29,  DNa  of  tobacco  regenerants  obtained  on  kanamycin;  lanes  30–34,  DNa  of  tobacco pants  regenerated  at  paromomycin;  C-,  negative  control;  C+,  positive  control;  M,  molecular  weight  marker  λ  DNa/ HindIII
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===Джерела===
* ''[[Nitovska I.O.]], [[Avilov I.D.]], [[Morgun B.V.]]'' The positive effect of antibiotic paromomycin compared with kanamycin for selection of transgenic plants with NPTII gene on the example of ''Nicotiana tabacum'' // Фактори експериментальної еволюції органiзмiв: Зб. наук. пр. / Національна академія наук України, Інститут молекулярної біології і генетикп, Укр. Т-во генетиків і селекціонерів iм. М.I. Вавилова; редкол. / В. А. Кунах (голов. ред.) [та iн.] – К.: Укр. т-во генетиків і селекціонерів ім. М.І. Вавилова, 2015. – Т. 17. – С. 270-273
* Доклад на [[Конференция «Факторы экспериментальной эволюции организмов» / 2015|X Международной научной конференции «Факторы экспериментальной эволюции организмов»]]

[[Category:Нітовська І.О.]]
[[Category:Моргун Б.В.]]
[[Category:Відділ молекулярної генетики]]